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Physiological functions of hemocytes newly emerged from the cultured hematopoietic organs in the silkworm, Bombyx mori
Author(s) -
Wang ChengLong,
Wang ZhiXiang,
Kariuki Michael M.,
Ling QingZhi,
Kiguchi Kenji,
Ling ErJun
Publication year - 2010
Publication title -
insect science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 45
eISSN - 1744-7917
pISSN - 1672-9609
DOI - 10.1111/j.1744-7917.2009.01288.x
Subject(s) - biology , hemolymph , hemocyte , haematopoiesis , bombyx mori , innate immune system , microbiology and biotechnology , immune system , insect , bombyx , immunology , propidium iodide , stem cell , apoptosis , programmed cell death , botany , biochemistry , gene
Cellular immunity is a very important part of insect innate immunity. It is not clear if hemocytes entering the hemolymph require a maturation process to become competent. The establishment of a tissue culture system for the insect hematopoietic organs would enable physiological function assays with hemocytes newly emerged from hematopoietic organs. To this end, we established a hematopoietic organ culture system for the purebred silkworm pnd pS and then studied the physiological functions of the newly emerged hemocytes. We found that Grace's medium supplemented with 10% heated silkworm larval plasma was better for culturing the hematopoietic organs of pnd pS . Newly emerged hemocytes phagocytosed propidium iodide‐labeled bacteria and encapsulated the Iml‐2 coated nickel beads as well as pupal tissue debris. This culture system is therefore capable of generating physiologically functional hemocytes. These hemocytes can be used to study the mechanisms of the hemocyte immune response among others.