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Isolation of microsatellite loci in the pollinating fig wasp of Ficus hispida, Ceratosolen solmsi
Author(s) -
Yu Hao,
Zhang TongXin,
Hu HaoYuan,
Niu LiMing,
Xiao Hui,
Zhang YanZhou,
Huang DaWei
Publication year - 2008
Publication title -
insect science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 45
eISSN - 1744-7917
pISSN - 1672-9609
DOI - 10.1111/j.1744-7917.2008.00214.x
Subject(s) - biology , microsatellite , loss of heterozygosity , ficus , primer (cosmetics) , rapd , genetics , polymerase chain reaction , allele , botany , gene , genetic diversity , chemistry , population , demography , organic chemistry , sociology
Microsatellite loci were isolated for Ceratosolen solmsi , pollinator of the dioecious Ficus hispida. We developed nine polymorphic microsatellite loci based on the method of polymerase chain reaction isolation of microsatellite arrays (PIMA). Enrichment of genomic libraries was performed by random amplified polymorphic DNA (RAPD). A subset of 38 positive clones was sequenced; 15 clones showed microsatellite loci. We tested 15 designed primer pairs and nine of them produced polymorphic amplification in 48 individual wasps collected from different fruits of the dioecious host fig Ficus hispida in China. Among the 48 individuals, 49 alleles were obtained at the nine loci. The observed heterozygosity ranged between 0.357 and 0.634.