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Partial genomic organization of ribosomal protein S7 gene from malaria vector Anopheles stephensi
Author(s) -
DIXIT RAJNIKANT,
DIXIT SARITA,
ROY UPAL,
SHOUCHE YOGESH S.,
GAKHAR SURENDRA
Publication year - 2007
Publication title -
insect science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 45
eISSN - 1744-7917
pISSN - 1672-9609
DOI - 10.1111/j.1744-7917.2007.00131.x
Subject(s) - biology , complementary dna , genetics , ribosomal protein , gene , coding region , intron , anopheles stephensi , untranslated region , microbiology and biotechnology , sequence analysis , nucleic acid sequence , ribosome , messenger rna , rna , aedes aegypti , botany , larva
In this study, we describe the partial genomic organization of ribosomal protein S7 gene isolated from the mosquito Anopheles stephensi . Initially a 558 bp partial cDNA sequence was amplified as precursor mRNA sequence containing 223 bp long intron. 5′ and 3′ end sequences were recovered using end specific rapid amplification of cDNA ends (RACE) polymerase chain reaction. The full‐length cDNA sequence was 914 nucleotide long with an open reading frame capable of encoding 192 amino acid long protein with calculated molecular mass of 22 174 Da and a pI point of 9.94. Protein homology search revealed > 75% identity to other insect's S7 ribosomal proteins. Analysis of sequence alignment revealed several highly conserved domains, one of which is related to nuclear localization signal (NLS) region of human rpS7 . Interestingly, intron nucleotide sequence comparison with A. gambiae showed a lesser degree of conservation as compared to coding and untranslated regions. Like this, early studies on the genomic organization and cDNA/ Expressed sequence tag analysis (EST) could help in genome annotation of A. stephensi , and would be likely to be sequenced in the future.