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Analysis of the structure and expression of the 30K protein genes in silkworm, Bombyx mori
Author(s) -
SUN QUAN,
ZHAO PING,
LIN YING,
HOU YONG,
XIA QINGYOU,
XIANG ZHONGHUAI
Publication year - 2007
Publication title -
insect science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 45
eISSN - 1744-7917
pISSN - 1672-9609
DOI - 10.1111/j.1744-7917.2007.00121.x
Subject(s) - bombyx mori , biology , gene , promoter , transcription (linguistics) , gene expression , bombyx , genetics , transcription factor , reverse transcription polymerase chain reaction , genome , microbiology and biotechnology , linguistics , philosophy
A group of lipoproteins with molecular sizes of approximately 30 kDa, referred to as 30K proteins, are synthesized in fat body cells in the fifth instar larvae of silkworm, Bombyx mori. Analyzing the silkworm genome and its expressed sequence tags (ESTs), we found 10 genes encoding 30K proteins, which are mainly distributed in three subfamilies. Of these, seven coding proteins were found to harbor the degrading sites of 30kP protease A, although the number of degrading sites may be different. As some potential core promoters and regulatory elements were supposed to be essential for gene transcription, the expression profiles of these genes were examined by semi‐quantitative reverse transcription polymerase chain reaction. Eight 30K protein genes were detected to express luxuriantly in the fat body, while two were hardly expressed. Such results suggest that these 30K proteins may have different functions, and their adjacent regulatory elements play a crucial role in regulating their transcription.