Premium
EXPRESSION OF A BEE‐VENOM PHOSPHOLIPASE A 2 FROM APIS CERANA CERANA IN ESCHERICHIA COLI
Author(s) -
Shen Lirong,
Cheng Jiaan,
Zhang Chuanxi
Publication year - 2004
Publication title -
insect science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 45
eISSN - 1744-7917
pISSN - 1672-9609
DOI - 10.1111/j.1744-7917.2004.tb00175.x
Subject(s) - apis cerana , biology , western blot , polyclonal antibodies , antigenicity , escherichia coli , microbiology and biotechnology , recombinant dna , biochemistry , botany , antibody , gene , genetics , honey bees
The venomous phospholipase A 2 (AcPLA 2 ) coding reading region of the Chinese honeybee ( Apis cerana cerana ), which is composed of 405 bp encoding a mature glycosylated peptide with 134 amino residues was transformed into the expression vector pETblue‐1. Then the recombinant vector was introduced into Escherichia coli Tuner (DE3) plac I for expression. Analysis result of SDS‐PAGE showed that the expression products had a protein band of about 15kD. Detection of western blot using ant‐European honeybee ( Apis mellifera ) phospholipase A 2 (AmPLA 2 ) polyclonal serum as the first antibody showed that the expression products appeared a special blot same as the native AmPLA 2 .The result demonstrated that the AcPLA 2 peptide had been expressed in E. coli and the AcPLA 2 has the similar antigenicity as the AmPLA 2 .