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CELLULAR LOCALIZATION AND EXPRESSION OF pygo DURING DROSOPHILA DEVELOPMENT
Author(s) -
Xinda LIN,
Xinhua LIN,
Jiaan CHENG
Publication year - 2003
Publication title -
insect science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 45
eISSN - 1744-7917
pISSN - 1672-9609
DOI - 10.1111/j.1744-7917.2003.tb00370.x
Subject(s) - biology , blastoderm , wnt signaling pathway , in situ hybridization , imaginal disc , microbiology and biotechnology , wing , signal transduction , embryo , drosophila (subgenus) , gene , gene expression , genetics , anatomy , drosophila melanogaster , embryogenesis , aerospace engineering , engineering
Wg/Wnt signaling is a key signaling pathway in Drosophila. Many genes involved in Wingless(wg) signal transduction pathway downstream of Wg, or it s vertebrate Wg homologue Wnt, have been identified. Transduction of the Wg signal downstream of Wg is mediated by nuclear TCF/LEF‐1, through association with Armadillo (Arm)β‐catenin. Pygopus (pygo) is a new identified component in this pathway. Cellular localization experiment showed that pygo was expressed specifically in the nucleus. The expression profile of pygo in embryos was examined using in situ hybridization. Although pygo expressed ubiquitously in the embryos, it expressed at relatively high level in pre‐blastoderm embryos which indicate a high degree of maternally provided message, followed by a low level of ubiquitous zygotic expression. This continues into larval tissues (including wing disc, eye disc and leg disc), where pygo appears to be expressed at low level. Comparison of pygo expression levels, in the wing disc, eye disc and leg disc, showed pygo expression level in the wing disc pouch and leg disc were relative higher.