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MOLECULAR MECHANISMS OF TARGET RESISTANCE IN THE ORGANOPHOSPHATE‐ AND PYRETHROID‐RE‐SISTANT HELICOVERPA ARMIGERA (HUBNER)
Author(s) -
Yuxin DONG,
Zhenhua TANG
Publication year - 2000
Publication title -
insect science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 45
eISSN - 1744-7917
pISSN - 1672-9609
DOI - 10.1111/j.1744-7917.2000.tb00342.x
Subject(s) - helicoverpa armigera , organophosphate , pyrethroid , strain (injury) , biology , knockdown resistance , microbiology and biotechnology , toxicology , pesticide , botany , larva , anatomy , cyfluthrin , agronomy
The molecular mechanisms of target resistance, i. e. acetyicholinesterase (AChE) and sodium channel insensitivity, in the organophosphate(0P)‐ and pyrethroid(Py)‐resistant (R) Helicoverpa armigera were investigated. The activity and V max of AChE from R strain were 1.09– and 1.23‐fold of the susceptible(S) strain respectively, but the K M value of AChE in R strain was only 0.67‐fold of S stain. The K i values of AChE from the R strain were 0.44 for DDVP and 0.50 for malathion respectively, as compared with those of the S strain. These data showed that the AChE from R strain might be qualitatively altered. The knockdown resistance ( kdr ) of the resistant H. armigera was also identified by PCR technique. The fragments of IIS6, linker of 11–111 and a part of IIS5‐IIs6 in the sodium channel were cloned and sequenced, and then compared to amino acid sequences from the R and S strains. and other insect species. There was no difference to be found at amino acid level in the above fragments cloned. It was suggested that the Rdr was not involved in the resistance of R strain.