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RAPD‐PCR DETECTION OF DNA POLYMORPHISM OF COTTON APHID *
Author(s) -
Peng GONG,
Xiaoxi ZHANG,
Xiaowen YANG,
Xiaofen CHEN
Publication year - 2000
Publication title -
insect science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 45
eISSN - 1744-7917
pISSN - 1672-9609
DOI - 10.1111/j.1744-7917.2000.tb00339.x
Subject(s) - biology , aphid , aphis gossypii , rapd , population , botany , host (biology) , genetic distance , aphididae , homoptera , horticulture , genetics , gene , genetic variation , genetic diversity , pest analysis , demography , sociology
The RAPDPCR technique was used to determine the DNA polymorphism of the aphid Aphis gossypii (Glover) collected from different host plants and in different seasons. Three primers were selected from 20 primers and used for cluster analysis based on the data of Nei's genetic distance (D). The results showed that the aphids on Chinese prickly ash differentiated obviously from the aphids on the other four host plants at DNA level. The seasonal population of cotton aphid might be clustered into three groups, i.e. the spring and autumn yellow colored aphids, the spring and autumn green colored aphids and the yellow dwarf form aphid in summer. However, the genetic relationship of dwarf form was more closely to the spring and autumn green colored aphid.