Premium
ULTRASTRUCTURE AND DNA RESTRICTION ENDONUCLEASE ANALYSIS OF CALLIPTAMUS ITALICUS ENTOMOPOXVIRUS
Author(s) -
Yongdan LI,
Liying WANG
Publication year - 2000
Publication title -
insect science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 45
eISSN - 1744-7917
pISSN - 1672-9609
DOI - 10.1111/j.1744-7917.2000.tb00231.x
Subject(s) - biology , cytoplasm , restriction enzyme , dna , budding , virus , ultrastructure , microbiology and biotechnology , endonuclease , morphology (biology) , virology , anatomy , genetics
Calliptamus italicus entomopoxvirus (CiEPV) was isolated from the grasshopper Calliptamus italicus . This virus mainly infects the host's fat body, and its development in the cytoplasm includes 4 steps: the appearance of viroplasm in the cytoplasm of fat body; the formation of immature virions by budding from viro‐plasm; the differentiation of mature virions from immature virions and the accumulation of occlusion‐body protein. The mature virions are oval with mulberry‐like surface, measuring 190 nm X 350 nm in size. Most of the occlusion bodies are elliptical, some are square‐ round in shape. There are great differences in size ranging from 1.5 μm X 1.9 μm to 8.8 μm X 12.3 μm. CiEPV DNA, when cleaved with restriction endonuclease Eco R I Bg 1 II and Hin d III, produces 16–18 and 10 fragments respectively, and the molecular weight of this DNA was estimated to be 135.7 X 10 6 daltons. Some characteristics of morphology and DNA were compared between CiEPV and Oedaleus asiaticus EPV (OaEPV) reported previously.