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PHYSIOLOGICAL AND BIOCHEMICAL MECHANISMS OF TEMPERATURE SHOCKS ON OVERWINTERING MATURE LARVAE OF PINE NEEDLE GALL MIDGE THECODIPLOSIS JAPONENSIS (DIPTERA: CE‐CIDOMYIIDAE) *
Author(s) -
Li Yiping,
Gong He,
Park HoYong
Publication year - 1999
Publication title -
insect science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 45
eISSN - 1744-7917
pISSN - 1672-9609
DOI - 10.1111/j.1744-7917.1999.tb00128.x
Subject(s) - midge , overwintering , biology , glutathione reductase , cold hardening , glutathione , desiccation , shock (circulatory) , larva , oxidative stress , supercooling , heat shock protein , gall , botany , horticulture , biochemistry , enzyme , glutathione peroxidase , medicine , thermodynamics , physics , gene
The supercooling points of cold (‐10C and ‐5C) and heat (37 C, 40 C and 45 C) shocked overwintering larvae were nearly the same as that of un‐shocked ones ( ca. ‐20C). Temperature shocks enhanced the ability to endure subzero temperature (‐ 15C, 3 h), and the cold shock treatment had more significant effect on maintaining larval survival than that of heat shock. It is the third insect that heat shock and cold shock enhanced its survival rate under low temperature simultaneously. A special stress protein (MW = 83 kD) was expressed under cold shock at ‐10 C and heat shock at 40 C or 45 C. It is also a few instances that a stress protein was expressed in the same insect under both heat shock and cold shock simultaneously. Meanwhile, the antioxidant system under different treatments was studied. Rapid cold hardening process had no oxidative stress because of the increase content of reduced glutathione and activity of glutathione reductase, but other treatments had.