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SINGLE ‐HELICOVERPA ARMIGERA TEST TO DETERMINE INSECTICIDE RESISTANCE GENOTYPE FREQUENCY *
Author(s) -
Qiao Chuanling,
Yan Yanchun
Publication year - 1998
Publication title -
insect science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 45
eISSN - 1744-7917
pISSN - 1672-9609
DOI - 10.1111/j.1744-7917.1998.tb00331.x
Subject(s) - propoxur , genotype , biology , acetylcholinesterase , aché , genotype frequency , helicoverpa armigera , population , allele frequency , enzyme , genetics , gene , biochemistry , larva , botany , pesticide , medicine , ecology , environmental health
A sensitive technique for identifying insecticide resistance genotype frequency of the Ace gene existing in natural populations of Helicoverpa armigera in China is described. The technique is based on the comparison of acetylcholinesterase (AChE) activity in 3 equal aliquots, which are taken from the homogenate of a single H. urnzigera: 1) in absence of inhibitor (well No. 1); 2) in absence of enzyme precursor (well No. 2); and 3) in presence of a concentration of propoxur inhibiting the AChE coded by the Ace S allele but not by the Ace R allele (well No. 3). An intensity of same strength in wells No. 1 and No. 3 indicates that propoxur has no effect on AChE activity, and the genotype should be Ace RR (when well No. 3 = well No. 1). A same weak intensity in well No. 2 and No. 3 (and a strong intensity in well No. l), indicates that propoxur completely inhibited AChE activity; and the genotype should be Ace Ss (when well No. 3 = well No. 2). Well No. 3 displaying an intermediate intensity between well No. 1 (strong intensity) and well No. 2 (weak intensity) indicates that AChE activity was partially inhibited by the propoxur concentration and genotype should be Ace Rs (when well No. 2