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BINDING ASSAY OF BACILLUS THURINGIENSIS CRY1AC INSECTICIDAL CRYSTAL PROTEINS IN DIAMONDBACK MOTH *
Author(s) -
Li Jianhong,
Akhurst Ray,
Yu Ziniu
Publication year - 1998
Publication title -
insect science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 45
eISSN - 1744-7917
pISSN - 1672-9609
DOI - 10.1111/j.1744-7917.1998.tb00330.x
Subject(s) - bacillus thuringiensis , diamondback moth , cry1ac , plutella , biology , brush border , midgut , larva , botany , vesicle , genetically modified crops , biochemistry , genetics , membrane , gene , transgene , bacteria
Binding assay was performed with NTP‐labeled Bacillus thuringiensis activated toxin Cry 1Ac on midgut brush border membrane vesicles (BBMVs) prepared from the whole larvae of dia‐mondback moth Plutella xylostella . We conclude that the BBMVs can be isolated properly and Cry 1Ac toxin binds saturably to BBMVs.