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EFFECT OF TANNIC ACID ON THE EFFECTIVENESS OF BACILLUlS THURINGIENSIS VAR. KURSTAKI AGAINST HELICOVERPA ARMIGERA (HÜBNER) *
Author(s) -
Wang Chenzhu,
Zhang Shufang,
Zhang Jihong,
Xiang Xiufeng
Publication year - 1997
Publication title -
insect science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 45
eISSN - 1744-7917
pISSN - 1672-9609
DOI - 10.1111/j.1744-7917.1997.tb00075.x
Subject(s) - tannic acid , helicoverpa armigera , bacillus thuringiensis , biology , bioassay , food science , botany , larva , bacteria , genetics
The role of tannic acid in increasing effectiveness of Bacillus thuringiensis var. kurstaki (HD‐1) against Helicover pa armigera was examined in bioassays on semisynthetic diet. Concentrations of B. thuringiensis (0 %, 0.005 % , 0. 01 %, 0.015 %, 0.02 %, 0.025 % wet weight) were incorporated into the diet containing 0. 025% tannic acid and tannic acid‐free diet. LD 50 of B. thuringiensis with tannic acid were 0.006% but that without tannic acid was 0.011%. Both B. thuringiensis and tannic acid retarded growth of H. armigera significantly, but there was no synergetic effect between them. Choice tests showed that B, thuringiensis deterred feeding of the fifth instar larvae of H. armigera but tannic acid had no such effect. Experiments on colony growth of B. thuringiensis on NBA media containing tannic acid (0, 1, 3, 6, 9, 12, 15, 18, 21 mg/100 ml) demonstrated that tannic acid reduced colony growth of B. thuringiensis , and inhibited sporulation above 15 mg/100 ml.