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BIOCHEMICAL ASPECTS OF INSECTICIDE RESISTANCE IN COTTON BOLLWORM FROM HANDAN OF HEBEI PROVINCE *
Author(s) -
Gao Xiwu,
Zhang Fan,
Zheng Bingzong,
Wang Rongjing,
Lin Bin
Publication year - 1996
Publication title -
insect science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.991
H-Index - 45
eISSN - 1744-7917
pISSN - 1672-9609
DOI - 10.1111/j.1744-7917.1996.tb00273.x
Subject(s) - methomyl , parathion , helicoverpa armigera , paraoxon , dose , biology , toxicology , bollworm , helicoverpa , carbamate , acetylcholinesterase , alkaline phosphatase , parathion methyl , biochemistry , pesticide , enzyme , pharmacology , botany , agronomy , larva
The cotton bollworms, Helicoverpa armigera Hübner, collected from Handan of Hebei Province, have evolved high resistance to pyrethroid, organophosphate and carbamate insecticides, The sensitivity of acetylcholinesterase (AChEs) to paraoxon and methomyl varied with the development stages of the cotton bollworm. After the treatments with LD 5 and LD 50 of parathion and methomyl to the cotton bollworms, the affinity of AChE to acetylthiocholine (ATCh) and acetyl‐β‐bmethyl‐thio choline (MeTCh) increased significantly except the treatment of parathion using LD 50 dosage while the sensitivity of AChEs to paraoxon significantly decreased. The sensitivity of AChEs to methomyl strongly increased in the treatment of parathion using LD 50 dosages while strongly decreased in other treatments. The affinity of carboxylesterase to β‐naphthyl acetate (β‐NA) was higher in groups of treatment with insecticides than in group of control. The glutathione‐s‐transferase (GST) activity significantly decreased in the induced groups using LD 5 dosages, while increased in the selection groups using LD 50 dosages. The effects of parathion and methomyl on the phosphatases of cotton bollworm were related to the dosages of application and the time after treatment and the effect on the alkaline phosphatase was stronger than on acid phosphatase.