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An Image Skeletonization‐Based Tool for Pollen Tube Morphology Analysis and Phenotyping F
Author(s) -
Wang Chaofeng,
Gui CaiPing,
Liu HaiKuan,
Zhang Dong,
Mosig Axel
Publication year - 2013
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/j.1744-7909.2012.01184.x
Subject(s) - skeletonization , pollen tube , pollen , segmentation , biology , context (archaeology) , morphology (biology) , phenotype , artificial intelligence , gene , botany , anatomy , computer science , genetics , paleontology , pollination
The mechanism underlying pollen tube growth involves diverse genes and molecular pathways. Alterations in the regulatory genes or pathways cause phenotypic changes reflected by cellular morphology, which can be captured using fluorescence microscopy. Determining and classifying pollen tube morphological phenotypes in such microscopic images is key to our understanding the involvement of genes and pathways. In this context, we propose a computational method to extract quantitative morphological features, and demonstrate that these features reflect morphological differences relevant to distinguish different defects of pollen tube growth. The corresponding software tool furthermore includes a novel semi‐automated image segmentation approach, allowing to highly accurately identify the boundary of a pollen tube in a microscopic image.