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Evaluation of Protein Extraction Methods for Vitis vinifera Leaf and Root Proteome Analysis by Two‐Dimensional Electrophoresis
Author(s) -
Jellouli Neila,
Salem Asma Ben,
Ghorbel Abdelwahed,
Jouira Hatem Ben
Publication year - 2010
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/j.1744-7909.2010.00973.x
Subject(s) - vitis vinifera , proteome , extraction (chemistry) , botany , biology , chemistry , chromatography , biochemistry
An efficient protein extraction method is crucial to ensure successful separation by two‐dimensional electrophoresis (2‐DE) for recalcitrant plant species, in particular for grapevine ( Vitis vinifera L.). Trichloroacetic acid‐acetone (TCA‐acetone) and phenol extraction methods were evaluated for proteome analysis of leaves and roots from the Tunisian cultivar ‘Razegui’. The phenol‐based protocol proved to give a higher protein yield, a greater spot resolution, and a minimal streaking on 2‐DE gels for both leaf and root tissues compared with the TCA‐based protocol. Furthermore, the highest numbers of detected proteins on 2‐DE gels were observed using the phenol extraction from leaves and roots as compared with TCA‐acetone extraction.