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Antisense‐mediated Depletion of Tomato Endoplasmic Reticulum Omega‐3 Fatty Acid Desaturase Enhances Thermal Tolerance
Author(s) -
Wang HuaSen,
Yu Chao,
Tang XianFeng,
Wang LiYan,
Dong XunChun,
Meng QingWei
Publication year - 2010
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/j.1744-7909.2010.00957.x
Subject(s) - endoplasmic reticulum , fatty acid desaturase , chloroplast , cauliflower mosaic virus , biology , transgene , organelle , fatty acid , genetically modified tomato , photosystem ii , genetically modified crops , biochemistry , microbiology and biotechnology , botany , gene , photosynthesis , polyunsaturated fatty acid
An endoplasmic reticulum‐localized tomato ω‐3 fatty acid desaturase gene ( LeFAD3 ) was isolated. The antisense tomato plants were obtained under the control of the cauliflower mosaic virus 35S promoter (35S‐CaMV). Northern blot analysis confirmed that the expression of LeFAD3 was inhibited in the tomato genome. Levels of 18:3 decreased and correspondingly levels of 18:2 increased in total lipids of leaves and roots. After heat stress, the fresh weight of the aerial parts of antisense transgenic plants was higher than that of the wild type (WT) plants. The membrane system ultrastructure of chloroplasts in leaf cells and all of the subcellular organelles in the root tips of transgenic plants remained more intact than those of WT. Relative electric conductivity increased less in transgenic plants than in WT. Under heat stress, the maximal photochemical efficiency of photosystem II (Fv/Fm) and the O 2 evolution rate decreased more in WT than in transgenic plants. These results suggested that the depletion of LeFAD3 increased the saturation of fatty acids and alleviated high temperature stress.