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Hydrogen Sulfide Promotes Root Organogenesis in Ipomoea batatas, Salix matsudana and Glycine max
Author(s) -
Zhang Hua,
Tang Jun,
Liu XiaoPing,
Wang Yun,
Yu Wei,
Peng WeiYan,
Fang Fang,
Ma DaiFu,
Wei ZhaoJun,
Hu LanYing
Publication year - 2009
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/j.1744-7909.2009.00885.x
Subject(s) - sodium hydrosulfide , ipomoea , auxin , glycine , chemistry , hydrogen sulfide , shoot , seedling , organogenesis , cutting , botany , horticulture , biochemistry , biology , sulfur , amino acid , organic chemistry , gene
In this report, we demonstrate that sodium hydrosulfide (NaHS), a hydrogen sulfide (H 2 S) donor, promoted adventitious root formation mediated by auxin and nitric oxide (NO). Application of the H 2 S donor to seedling cuttings of sweet potato ( Ipomoea batatas L.) promoted the number and length of adventitious roots in a dose‐dependent manner. It was also verified that H 2 S or HS − rather than other sulfur‐containing components derived from NaHS could be attributed to the stimulation of adventitious root formation. A rapid increase in endogenous H 2 S, indole acetic acid (IAA) and NO were sequentially observed in shoot tips of sweet potato seedlings treated with HaHS. Further investigation showed that H 2 S‐mediated root formation was alleviated by N‐1‐naphthylphthalamic acid (NPA), an IAA transport inhibitor, and 2‐(4‐carboxyphenyl)‐4,4,5,5‐tetramethylimidazoline‐1‐oxyl‐3‐oxide (cPTIO), an NO scavenger. Similar phenomena in H 2 S donor‐dependent root organogenesis were observed in both excised willow ( Salix matsudana var. tortuosa Vilm) shoots and soybean ( Glycine max L.) seedlings. These results indicated that the process of H 2 S‐induced adventitious root formation was likely mediated by IAA and NO, and that H 2 S acts upstream of IAA and NO signal transduction pathways.