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Endogenous Hydrogen Peroxide Plays a Positive Role in the Upregulation of Heme Oxygenase and Acclimation to Oxidative Stress in Wheat Seedling Leaves
Author(s) -
Chen XiaoYue,
Ding Xiao,
Xu Sheng,
Wang Ren,
Xuan Wei,
Cao ZeYu,
Chen Jian,
Wu HongHong,
Ye MaoBing,
Shen WenBiao
Publication year - 2009
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/j.1744-7909.2009.00869.x
Subject(s) - heme oxygenase , endogeny , superoxide dismutase , oxidative stress , downregulation and upregulation , chemistry , lipid peroxidation , hydrogen peroxide , heme , acclimatization , superoxide , catalase , oxygenase , biochemistry , microbiology and biotechnology , enzyme , biology , gene , botany
Pretreatment of lower H 2 O 2 doses (0.05, 0.5 and 5 mM) for 24 h was able to dose‐dependently attenuate lipid peroxidation in wheat seedling leaves mediated by further oxidative damage elicited by higher dose of H 2 O 2 (150 mM) for 6 h, with 0.5 mM H 2 O 2 being the most effective concentrations. Further results illustrated that 0.5 mM H 2 O 2 pretreatment triggered the biphasic production of H 2 O 2 during a 24 h period. We also noticed that only peak I (0.25 h) rather than peak II (4 h) was approximately consistent with the enhancement of heme oxygenase (HO) activity, HO‐1 gene expression. Meanwhile, enhanced superoxide dismutase (SOD) activity, Mn‐SOD and Cu,Zn‐SOD transcripts might be a potential source of peak I of endogenous H 2 O 2 . Further results confirmed that 0.5 mM H 2 O 2 treatment for 0.5 h was able to upregulate HO gene expression, which was detected by enzyme activity determination, semi‐quantitative reverse transcription‐polymerase chain reaction and western blotting. Meanwhile, the application of N,N’‐dimethylthiourea, a trap for endogenous H 2 O 2 , not only blocked the upregulation of HO, but also reversed the corresponding oxidation attenuation. Together, the above results suggest that endogenous H 2 O 2 production (peak I) plays a positive role in the induction of HO by enhancing its mRNA level and protein expression, thus leading to the acclimation to oxidative stress.