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Characterization of Kiwifruit Xyloglucan
Author(s) -
Li Xingjun,
Sakurai Naoki,
Nevins Donald J.
Publication year - 2009
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/j.1744-7909.2009.00862.x
Subject(s) - xyloglucan , actinidia deliciosa , chemistry , xylose , actinidia , gel permeation chromatography , cell wall , fucose , actinidia chinensis , chromatography , polysaccharide , sugar , hemicellulose , size exclusion chromatography , arabinose , galactose , biochemistry , botany , biology , organic chemistry , enzyme , fermentation , hydrolysis , polymer
Structural characteristics of xyloglucan are constant in the pericarp cell walls of kiwifruit ( Actinidia deliciosa ) throughout fruit enlargement and maturation. Most of the xyloglucan (XG) persists in the cell walls of ripe kiwifruit. XG from the pericarp tissues of 36‐h ethylene‐treated kiwifruit was extracted as hemicellulose II (HC‐II) with 4.28 M KOH containing 0.02% NaBH 4 , and purified using iodine precipitation and subsequent anion‐exchange chromatography. This purifying protocol increased XG purity from 50 mol% in HC‐II fraction to 62 mol% in the purified XG powder. The molar ratio of glucose: xylose: galactose: fucose in the purified XG was 10: 6.9: 2.1: 0.3. Gel permeation chromatography indicated that purified XG had an average molecular‐mass of 161 KDa, a value that exceeds the 95 KDa M r determined for total polymeric sugars. Sugar linkage analysis confirmed the lack of fucose in the kiwifruit XG, but a small amount of arabinoxylan and low M r glucomannan remained associated with this fraction.