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UV‐B‐induced Oxidative Damage and Protective Role of Exopolysaccharides in Desert Cyanobacterium Microcoleus vaginatus
Author(s) -
Chen LanZhou,
Wang GaoHong,
Hong Song,
Liu An,
Li Cheng,
Liu YongDing
Publication year - 2009
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/j.1744-7909.2008.00784.x
Subject(s) - reactive oxygen species , sucrose , lipid peroxidation , malondialdehyde , chemistry , photosynthesis , oxidative phosphorylation , dna damage , oxidative stress , biochemistry , botany , biology , dna
UV‐B‐induced oxidative damage and the protective effect of exopolysaccharides (EPS) in Microcoleus vaginatus , a cyanobacterium isolated from desert crust, were investigated. After being irradiated with UV‐B radiation, photosynthetic activity ( Fv/Fm ), cellular total carbohydrates, EPS and sucrose production of irradiated cells decreased, while reducing sugars, reactive oxygen species (ROS) generation, malondialdehyde (MDA) production and DNA strand breaks increased significantly. However, when pretreated with 100 mg/L exogenous EPS, EPS production in the culture medium of UV‐B stressed cells decreased significantly; Fv/Fm , cellular total carbohydrates, reducing sugars and sucrose synthase (SS) activity of irradiated cells increased significantly, while ROS generation, MDA production and DNA strand breaks of irradiated cells decreased significantly. The results suggested that EPS exhibited a significant protective effect on DNA strand breaks and lipid peroxidation by effectively eliminating ROS induced by UV‐B radiation in M. vaginatus .