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GAMETOPHYTIC FACTOR 1 , Involved in Pre‐mRNA Splicing, Is Essential for Megagametogenesis and Embryogenesis in Arabidopsis
Author(s) -
Liu Man,
Yuan Li,
Liu NaiYou,
Shi DongQiao,
Liu Jie,
Yang WeiCai
Publication year - 2009
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/j.1744-7909.2008.00783.x
Subject(s) - arabidopsis , biology , snrnp , rna splicing , biogenesis , genetics , gene , microbiology and biotechnology , mutant , alternative splicing , splicing factor , rna , messenger rna
RNA biogenesis is essential and vital for accurate expression of genes. It is obvious that cells cannot continue normal metabolism when RNA splicing is interfered with. sgt13018 is such a mutant, with partial loss of function of GAMETOPHYTIC FACTOR 1 ( GFA1 ); a gene likely involved in RNA biogenesis in Arabidopsis . The mutant is featured in the phenotype of diminished female gametophyte development at stage FG5 and is associated with the arrest of early embryo development in Arabidopsis . Bioinformatics data showed that homologs of gene GFA1 in yeast and human encode putative U5 snRNP‐specific proteins required for pre‐mRNA splicing. Furthermore, the result of yeast two‐hybrid assay indicated that GFA1 physically interacted with AtBrr2 and AtPrp8, the putative U5 snRNP components, of Arabidopsis . This investigation suggests that GFA1 is involved in mRNA biogenesis through interaction with AtBrr2 and AtPrp8 and functions in megagametogenesis and embryogenesis in plant.