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Crossability Barriers in the Interspecific Hybridization between Oryza sativa and O. meyeriana
Author(s) -
Fu XueLin,
Lu YongGen,
Liu XiangDong,
Li JinQuan
Publication year - 2009
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/j.1744-7909.2008.00728.x
Subject(s) - oryza sativa , interspecific competition , interspecific hybridization , biology , botany , hybrid , genetics , gene
Oryza meyeriana Baill (GG genome) is a precious germplasm in the tertiary gene pool of cultivated rice (AA genome), and possesses important traits such as resistance and tolerance to biotic and abiotic stress. However, interspecific crossability barrier, a critical bottleneck restricting genes transfer from O . meyeriana to cultivars has led to no hybrids through conventional reproduction. Therefore, the reasons underlying incrossability were investigated in the present report. The results showed that: (i) at 3–7 d after pollination (DAP), many hybrid embryos degenerated at the earlier globular‐shaped stage, and could not develop into the later pear‐shaped stage. Meanwhile, free endosperm nuclei started to degenerate at 1 DAP, and cellular endosperm could not form at 3 DAP, leading to nutrition starvation for young embryo development; (ii) at 11–13 DAP, almost all hybrid ovaries aborted. Even though 72.22% of hybrid young embryos were produced in the interspecific hybridization between O . sativa and O . meyeriana, young embryos were not able to further develop into hybrid plantlets via culturing in vitro . The main reason for the incrossability was hybrid embryo inviability, presenting as embryo development stagnation and degeneration since 3 DAP. Some possible approaches to overcome the crossability barriers in the interspecific hybridization between O . sativa and O. meyeriana are discussed.