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Location of Caspase 3‐like Protease in the Development of Sieve Element and Tracheary Element of Stem in Cucurbita moschata
Author(s) -
Hao Xia,
Qian Jie,
Xu Shan,
Song Xin,
Zhu Jian
Publication year - 2008
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/j.1744-7909.2008.00719.x
Subject(s) - immunoelectron microscopy , protease , biology , microbiology and biotechnology , phloem , immunogold labelling , sieve tube element , botany , ultrastructure , biochemistry , immunohistochemistry , enzyme , immunology
The casepase is considered to regulate the process of programmed cell death in the development of organisms. In this study, caspase 3‐like protease was detected by immunohistochemistry and immunoelectron microscopy during the development of sieve element and tracheary element of stem in Cucurbita moschata Duch. Antibody with brown color (under light microscopy) and gold particles (under transmission electron microscopy) for detecting caspase 3‐like protease was mainly displayed in inner phloem, external phloem and xylem in the region close to procambium. From the results it was considered that caspase 3‐like protease did exist in vascular elements and played different roles during the development of sieve and tracheary elements, and different types of programmed cell death might be carried out. The caspase 3‐like protease mainly participated in making cytoplasmic streaming cease and in degrading P‐protein bodies; however, it rarely participated in the function for signal transferring in the developmental sieve element. However, it might induce calcium accumulation for rupturing the tonoplast in the signal of PCD in the developmental tracheary element.