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In vitro Transient Expression System of Latex C‐serum was used for Analysis of Hevein Promoter in Response to Abscisic Acid in Hevea brasiliensis
Author(s) -
Fei XiaoWen,
Deng XiaoDong
Publication year - 2008
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/j.1744-7909.2007.00628.x
Subject(s) - hevea brasiliensis , abscisic acid , hevea , gene , natural rubber , gene expression , transgene , biology , in vitro , microbiology and biotechnology , botany , chemistry , genetics , organic chemistry
Hevein has been found to be an essential element in coagulation of rubber particles in latex of rubber trees. In a previous study, we cloned a 1 241‐bp fragment of a 5′ upstream region of the hevein gene by genome walking. This fragment was analyzed by a 5′ end nested deletion method in the present study, fused with a uidA ( gus ) gene to produce a series of tested constructs, which were transferred into C‐serum of latex and the Gus activities were detected. Results showed that the fragment from −749 to −292 was sufficient for expression of gus gene in latex, and the fragment from −292 to −168 was crucial in response to abscisic acid inducement. In a transient transgenic test of rubber leaf with particle bombardment, construct Hev749 conferred gus ‐specific expression in veins, in which the latex tubes mainly distributed. This implies that the fragment from −749 to −292 was laticiferous‐specific.