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High‐throughput Binary Vectors for Plant Gene Function Analysis
Author(s) -
Lei ZhiYong,
Zhao Ping,
Cao MinJie,
Cui Rong,
Chen Xi,
Xiong LiZhong,
Zhang QiFa,
Oliver David J.,
Xiang ChengBin
Publication year - 2007
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/j.1744-7909.2007.00442.x
Subject(s) - cloning (programming) , multiple cloning site , integrase , computational biology , vector (molecular biology) , gene , plasmid , biology , function (biology) , gene silencing , throughput , genetics , computer science , recombinant dna , telecommunications , wireless , programming language
A series of high‐throughput binary cloning vectors were constructed to facilitate gene function analysis in higher plants. This vector series consists of plasmids designed for plant expression, promoter analysis, gene silencing, and green fluorescent protein fusions for protein localization. These vectors provide for high‐throughput and efficient cloning utilizing sites for λ phage integrase/excisionase. In addition, unique restriction sites are incorporated in a multiple cloning site and enable promoter replacement. The entire vector series are available with complete sequence information and detailed annotations and are freely distributed to the scientific community for non‐commercial uses.