Premium
CO 2 Exchange and Chlorophyll Fluorescence of Phospho‐enolpyruvate Carboxylase Transgenic Rice Pollen Lines
Author(s) -
Ling LiLi,
Lin HongHui,
Ji BenHua,
Jiao DeMao
Publication year - 2006
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/j.1744-7909.2006.00376.x
Subject(s) - phosphoenolpyruvate carboxylase , photosynthesis , chlorophyll fluorescence , photosystem ii , oryza sativa , biology , pollen , botany , genetically modified rice , chlorophyll , rubisco , transgene , biochemistry , genetically modified crops , gene
To elucidate the photosynthetic physiological characteristics and the physiological inherited traits of rice (Oryza sativa L.) hybrids and their parents, physiological indices of photosynthetic CO 2 exchange and chlorophyll fluorescence parameters were measured in leaves of the maize phosphoenolpyruvate carboxylase (PEPC) transgenic rice as the male parent, sp. japonica rice cv. 9516 as the female parent, and the stable JAAS45 pollen line. The results revealed that the PEPC gene could be stably inherited and transferred from the male parent to the JAAS45 pollen line. Moreover, the JAAS45 pollen line exhibited high levels of PEPC activity, manifesting higher saturated photosynthetic rates, photosynthetic apparent quantum yield (AQY), photochemical efficiency of photosystem II and photochemical and non‐photochemical quenching, which indicated that the JAAS45 pollen line has a high tolerance to photo‐inhibition/photo‐oxidation under strong light and high temperature. Furthermore, JAAS45 was confirmed to still be a C 3 plant by δ13C carbon isotope determination and was demonstrated to have a limited photosynthetic C 4 microcycle by feeding with exogenous C 4 primary products, such as oxaloacetate or malate, or phosphoenolpyruvate. The present study explains the physiological inherited properties of PEPC transgenic rice and provides an expectation for the integration of traditional breeding and biological technology. (Managing editor: Ping He)