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A Novel Approach to Functional Analysis of the Ribulose Bisphosphate Carboxylase Small Subunit Gene by Agrobacterium ‐Mediated Gene Silencing
Author(s) -
Zhou XiaoFu,
Ma PengDa,
Wang RenHou,
Liu Bo,
Wang XingZhi
Publication year - 2006
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/j.1744-7909.2006.00320.x
Subject(s) - nicotiana benthamiana , gene silencing , biology , gene , gene expression , protein subunit , microbiology and biotechnology , agrobacterium , rubisco , genetics , transgene
A novel approach to virus‐induced post‐transcriptional gene silencing for studying the function of the ribulose bisphosphate carboxylase small subunit (rbcS) gene was established and optimized using potato virus X vector and Nicotiana benthamiana as experimental material. The analysis of silencing phenomena, transcriptional level, protein expression, and pigment measurement showed that the expression of the rbcS endogenous gene was inactivated by the expression of a 500‐bp homologous cDNA fragment carried in the virus vector.