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Effects of Exogenous Spermidine on Photosystem II of Wheat Seedlings Under Water Stress
Author(s) -
Duan HuiGuo,
Yuan Shu,
Liu WenJuan,
Xi DeHui,
Qing DongHong,
Liang HouGuo,
Lin HongHui
Publication year - 2006
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/j.1744-7909.2006.00315.x
Subject(s) - photosystem ii , spermidine , chemistry , photosynthesis , lipid peroxidation , membrane permeability , membrane , biophysics , horticulture , biochemistry , biology , oxidative stress , enzyme
The effects of exogenous spermidine (Spd) on lipid peroxidation, relative plasma membrane permeability, photosystem II (PSII) gene expression and PSII photochemical activity in water‐stressed wheat seedlings were investigated. The decrease in relative water content (RWC), Chl content, and 2,6‐dichlorophenol indophenol (DCIP) photoreduction of PSII, and increases in electrolyte leakage of plasma membranes and malonyldialdehyde (MDA) in water‐stressed leaves was alleviated by Spd pretreatment. Furthermore, Western and Northern blot analysis showed that decreases in the PSII major proteins D1, D2 and LHCII and the transcripts of corresponding genes psbA, psbD and cab were also alleviated by Spd pretreatment under water stress. These results suggest that the application of exogenous Spd protects PSII against water stress at both the transcriptional level and the translational level, and allows PSII to retain a higher activity level during water stress. The protective role of Spd in the photosynthetic apparatus also is discussed. (Managing editor: Ping He)