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Comparative Analysis of the Endosperm Proteins Separated by 2‐D Electrophoresis for Two Cultivars of Hybrid Rice (Oryza sativa L.)
Author(s) -
Yang Pingfang,
Shen Shihua,
Kuang Tingyun
Publication year - 2006
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/j.1744-7909.2006.00312.x
Subject(s) - endosperm , oryza sativa , cultivar , storage protein , biology , starch , botany , starch synthase , horticulture , chemistry , biochemistry , amylose , gene , amylopectin
Liangyoupeijiu is a two‐parental‐line, and Shanyou63 is a three‐parental‐line hybrid rice ( Oryza sativa L.). Although both belong to the indica subspecies, they have obvious differences with respect to morphology, physiology and grain quality. Variations in endosperm protein compositions were studied by comparing the 2‐D electrophoresis (2‐DE) maps for these two cultivars of hybrid rice. After matrix‐assisted laser desorption/ionization time of flight mass spectrometry (MALDI‐TOF/MS) analysis, a 21‐kDa precursor of 19‐kDa globulin was identified as the major storage protein for both cultivars. Some isoforms of peroxiredoxin and seed maturation protein were found to only exist in Shanyou63, whereas aldose reductase and starch granule‐bound starch synthase were only detected in Liangyoupeijiu. These data might provide a foundation for further comparative studies of these two cultivars of hybrid rice. (Managing editor: Ping He)