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Petunia Germinating Pollen S/D3 Interacts with S‐RNases in Petunia hybrida Vilm.
Author(s) -
Guo YanXia,
Zhang YanSheng,
Xue YongBiao
Publication year - 2006
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/j.1744-7909.2006.00285.x
Subject(s) - petunia , biology , pollen , genetics , gynoecium , solanaceae , botany , gene , stamen
Self‐incompatibility (SI) is a genetic mechanism of self/non‐self pollen recognition to prevent self‐fertilization in many flowering plants and, in most cases, this is controlled by a multi‐allelic S‐locus. S‐RNase and S‐locus F box (SLF) proteins have been shown to be the female and male determinants of gametophytic self‐incompatibility (GSI), respectively, in the Solanaceae, Scrophulariaceae and Rosaceae. Nevertheless, it is thought that additional factors are required for the SI response. Herein, we constructed a mature anther cDNA library from a self‐incompatible Petunia hybrida Vilm. line of the S3S3 haplotype. Using AhS2‐RNase from Antirrhinum hispanicum as a bait for yeast two‐hybrid screening, we found that petunia germinating pollen (PGP) S/D3 was capable of interacting physically with the bait. However, the interaction lacked haplotype specificity. The PGPS/D3 gene is a single copy gene that is expressed in tissues such as the style, ovary, pollen, and leaf. The PGPS/D3::GFP (green fluorescence protein) construct was detected in both the membrane and cytoplasm. The implications of these findings in the operation of S‐RNase‐based SI are discussed. (Managing editor: Li‐Hui Zhao)