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Application of Non‐invasive Microsensing System to Simultaneously Measure Both H + and O 2 Fluxes Around the Pollen Tube
Author(s) -
Xu Yue,
Sun Tong,
Yin LiPing
Publication year - 2006
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/j.1744-7909.2006.00281.x
Subject(s) - pollen tube , pollen , proton , measure (data warehouse) , ion , extracellular , electrode , biophysics , tube (container) , chemistry , analytical chemistry (journal) , physics , materials science , botany , biology , chromatography , biochemistry , nuclear physics , computer science , composite material , pollination , organic chemistry , database
Various ionic and molecular activities in the extracellular environment are vital to plant cell physiological processes. A noninvasive microsensing system (NMS) based on either the scanning ion‐selective electrode technique (SIET) or the scanning polarographic electrode technique (SPET) is able to obtain information regarding the transportation of various ions/molecules in intact samples under normal physiological conditions. The two‐probe simultaneous test system (2STS) is an integrated system composed of SIET, SPET, and a Xu‐Kunkel sampling protocol. In the present study, 2STS was able to simultaneously measure fluxes of H + and O 2 of the lily ( Lilium longiflorum Thunb. cv. Ace) pollen tube while avoiding interference between the two probes. The results indicate that the proton fluxes were effluxes, whereas the oxygen fluxes were influxes, and they were closely correlated to each other surrounding the constitutive alkaline band region. Specifically, when the proton effluxes increased, the oxygen influxes also increased. Therefore, the hypothesis of condensed active mitochondria existing in the alkalized area of the pollen tube proposed by Hepler's group is supported. (Managing editor: Wei Wang)