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Carbon Monoxide Alleviates Salt‐Induced Oxidative Damage in Wheat Seedling Leaves
Author(s) -
Huang BenKai,
Xu Sheng,
Xuan Wei,
Li Ming,
Cao ZeYu,
Liu KaiLi,
Ling TengFang,
Shen WenBiao
Publication year - 2006
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/j.1744-7909.2006.00220.x
Subject(s) - catalase , chemistry , seedling , lipid peroxidation , superoxide dismutase , oxidative stress , peroxidase , heme , hemoglobin , biochemistry , reactive oxygen species , horticulture , biology , enzyme
Carbon monoxide (CO), a by‐product released during the degradation of heme by heme oxygenases (EC 1.14.99.3) in animals, is regarded as an important physiological messenger or bioactive molecule involved in many biological events that has been recently reported as playing a major role in mediating the cytoprotection against oxidant‐induced lung injury. In the present study, we first determined the protective effect of exogenous CO against salt‐induced oxidative damage in wheat seedling leaves. Wheat seedlings treated with 0.01 μmol/L hematin as the CO donor demonstrated significant reversal of chlorophyll decay, dry weight, and water loss induced by 300 mmol/L NaCl stress. Interestingly, the increase in lipid peroxidation observed in salt‐treated leaves was reversed by 0.01 nmol/L hematin treatment. Time‐course analyses showed that application of 0.01 μmol/L hematin enhanced guaiacol peroxidase, superoxide dismutase, ascorbate peroxidase and catalase activities in wheat seedling leaves subjected to salt stress. These effects are specific for CO because the CO scavenger hemoglobin (1.2 mg/L) blocked the actions of the CO donor hematin. However, higher concentration of the CO donor (1.0 μmol/L) did not alleviate dry weight and water loss of salt‐stressed wheat seedlings. These results suggest that exogenous application of low levels of a CO donor may be advantageous against salinity toxicity. (Managing editor: Ping He)