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PCR‐RFLP and AP‐PCR of rbc L and ITS of rDNA Show That × Taxodiomeria peizhongii ( Taxodium × Cryptomeria ) Is not an Intergeneric Hybrid
Author(s) -
Ling Yan,
Lu WeiFeng,
Lu Fan,
Wang YuGuo,
Chen JiaKuan,
Zhang WenJu
Publication year - 2006
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/j.1744-7909.2006.00209.x
Subject(s) - taxodium , restriction fragment length polymorphism , biology , cryptomeria , microbiology and biotechnology , polymerase chain reaction , gene , ribosomal rna , botany , genetics , japonica
× Taxodiomeria peizhongii Z. J. Ye, J. J. Zhang et S. H. Pan was regarded as a new intergeneric hybrid between Taxodium mucronatum Tenore (as the female donor) and Cryptomeria fortunei Hooibrenk ex Otto et Dietr (as the male donor). To confirm the authenticity of the intergeneric hybrid, we analyzed the rbc L gene and the internal transcribed spacer (ITS) of 26S‐18S ribosomal RNA gene of the three species using polymerase chain reaction‐restriction fragment length polymorphism (PCR‐RFLP) and arbitrarily primed PCR (AP‐PCR), and obtained the following results: i) Taxodiomeria peizhongii had the same RFLP maps of the rbc L gene and the ITS as Taxodium mucronatum , but was different from C. fortune i; ii) a 311‐bp PCR amplification product was obtained in C. fortunei by AP‐PCR of ITS, but was not found in Taxodiomeria peizhongii. Our results have demonstrated that C. fortunei did not provide any genome for Taxodiomeria peizhongii , implying that T. peizhongii is not an intergeneric hybrid between the two species. (Managing editor: Wei Wang)