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Karyotyping of Brassica napus L. Based on C 0 t ‐1 DNA Banding by Fluorescence In Situ Hybridization
Author(s) -
WEI WenHui,
ZHAO WanPeng,
WANG LiJun,
CHEN Bo,
LI YunChang,
SONG YunChun
Publication year - 2005
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/j.1744-7909.2005.00186.x
Subject(s) - karyotype , fluorescence in situ hybridization , brassica , biology , in situ , fluorescence , microbiology and biotechnology , botany , chromosome , genetics , chemistry , physics , gene , optics , organic chemistry
In order to precisely recognize and karyotype Brassica napus L. chromosomes, C 0 t ‐1 DNA was extracted from its genomic DNA, labeled withbiotin‐11‐dUTP and in situ hybridized. The hybridized locations were detected by Cy3‐conjugated streptavidin. Specific fluorescence in situ hybridization (FISH) signal bands were detected on all individual chromosome pairs. Each chromosome pair showed specific banding patterns. The B. napus karyotype has been constructed, for the first time, on the basis of both C 0 t ‐1 DNA FISH banding patterns and chromosome morphology. (Managing editor: Wei WANG)