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Effects of the Application of Different Concentrations of NaN 3 for Different Times on the Morphological and Cytogenetic Characteristics of Barley ( Hordeum vulgare L.) Seedlings
Author(s) -
ILBAS Ali Irfan,
EROGLU Yasemin,
EROGLU Halil Erhan
Publication year - 2005
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/j.1744-7909.2005.00137.x
Subject(s) - hordeum vulgare , mitotic index , coleoptile , germination , sodium azide , biology , hordeum , horticulture , mitosis , botany , poaceae , biochemistry , microbiology and biotechnology
Sodium azide (NaN 3 ) has been used in many biological studies as a mutagen. In the present study, the morphological and cytogenetic effects of NaN 3 on barley (Hordeum vulgare L.) seedlings were investigated. Seeds of barley were treated with different concentrations of NaN 3 (0.5, 1.0, 1.5, 2.0, 2.5, and 3.0 mmol/L) and applied for different periods of time (3 and 4 h). Parameters investigated, except for the mitotic index, were determined on Days 7 and 14. Increasing concentrations of NaN 3 affected germination rates on Days 7 and 14 following application for 3 and 4 h. Although the length of the roots and leaves was affected by treatment with NaN 3 on Day 14 of the germination period, coleoptile length was affected by NaN 3 treatment on Day 7. Increasing concentrations of NaN 3 and increased treatment period decreased the mitotic index compared with the untreated control. The inhibitory effects of NaN 3 on the mitotic index indicate that NaN 3 can have genotoxic and mutagenic effects on barley seedlings. (Managing editor: Ping HE)