Premium
Impacts of Elevated CO 2 Concentration on Biochemical Composition, Carbonic Anhydrase, and Nitrate Reductase Activity of Freshwater Green Algae
Author(s) -
XIA JianRong,
GAO KunShan
Publication year - 2005
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/j.1744-7909.2005.00114.x
Subject(s) - nitrate reductase , chlorella pyrenoidosa , chlamydomonas reinhardtii , carbonic anhydrase , green algae , nitrate , algae , chemistry , biochemistry , composition (language) , chlorophyceae , biology , chlorella , chlorophyta , botany , enzyme , ecology , linguistics , philosophy , gene , mutant
To investigate the biochemical response of freshwater green algae to elevated CO 2 concentrations, Chlorella pyrenoidosa Chick and Chlamydomonas reinhardtii Dang cells were cultured at different CO 2 concentrations within the range 3‐186 ümol/L and the biochemical composition, carbonic anhydrase (CA), and nitrate reductase activities of the cells were investigated. Chlorophylls (Chl), carotenoids, carbonhydrate, and protein contents were enhanced to varying extents with increasing CO 2 concentration from 3‐186 ümol/L. The CO 2 enrichment significantly increased the Chl a/Chl b ratio in Chlorella pyrenoidosa , but not in Chlamydomonas reinhardtii. The CO 2 concentration had significant effects on CA and nitrate reductase activity. Elevating CO 2 concentration to 186 ümol/L caused a decline in intracellular and extracellullar CA activity. Nitrate reductase activity, under either light or dark conditions, in C. reinhardtii and C. pyrenoidosa was also significantly decreased with CO 2 enrichment. From this study, it can be concluded that CO 2 enrichment can affect biochemical composition, CA, and nitrate reductase activity, and that the biochemical response was species dependent. (Managing editor: Ping HE)