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Promotive Effect of Low Concentrations of NaHSO 3 on Photophosphorylation and Photosynthesis in Phosphoenolpyruvate Carboxylase Transgenic Rice Leaves
Author(s) -
JI BenHua,
TAN HongHe,
ZHOU Rong,
JIAO DeMao,
SHEN YunGang
Publication year - 2005
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/j.1744-7909.2005.00033.x
Subject(s) - phosphoenolpyruvate carboxylase , genetically modified rice , photosynthesis , photophosphorylation , oryza sativa , transgene , biochemistry , phosphoenolpyruvate carboxykinase , chemistry , biology , botany , enzyme , genetically modified crops , chloroplast , gene
Spraying a 1–2 mmol/L solution of NaHSO 3 on the leaves of wild‐type rice ( Oryza sativa L.) Kitaake (WT), phosphoenolpyruvate carboxylase (PEPC) transgenic (PC) rice and PEPC+phosphate dikinase (PPDK) transgenic rice (PC+PK), in which the germplasm was transformed with wild‐type Kitaake as the gene receptor, resulted in an enhancement of the net photosynthetic rate by 23.0%, 28.8%, and 34.4%, respectively, for more than 3 d. It was also observed that NaHSO 3 application caused an increase in the ATP content in leaves. Spraying PMS (a cofactor catalysing the photophosphorylation cycle) and NaHSO 3 separately or together on leaves resulted in an increase in photosynthesis with all treatments. There was no additional effect on photosynthetic rate when the mixture was applied, suggesting that the mechanism by which NaHSO 3 promotes photosynthesis is similar to the mechanism by which PMS acts and that both of compounds enhanced the supply of ATP. After spraying a solution of NaHSO 3 on leaves, compared with the WT Kitaake rice, a greater enhancement of net photosynthetic rate was observed in PEPC transgenic (PC) and PEPC+PPDK transgenic (PC+PK) rice, with the greatest increase being observed in the latter group. Therefore ATP supply may become the limiting factor that concentrates CO 2 in rice leaves transformed with an exogenous PEPC gene and exogenous PEPC+PPDK genes. (Managing editor: Ping HE)