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Cloning, Physical Mapping and Expression Analysis of a Wheat Mlo ‐like Gene
Author(s) -
YU Ling,
NIU JiShan,
CHEN PeiDu,
MA ZhengQiang,
LIU DaJun
Publication year - 2005
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/j.1744-7909.2005.00030.x
Subject(s) - biology , complementary dna , gene , blumeria graminis , microbiology and biotechnology , hordeum vulgare , southern blot , genbank , blot , gene expression , genetics , northern blot , sequence analysis , botany , plant disease resistance , poaceae
Reverse transcription‐polymerase chain reaction (RT‐PCR) was performed to isolate a cDNA clone using specific primers designed based on the barley ( Hordeum vulgare L.) Mlo gene cDNA sequence. A full‐length cDNA encoding an Mlo‐like protein was isolated and characterized in a Triticum aestivum L.‐ Haynaldia villosa L. 6VS/6AL translocation line. The putative protein consists of 534 amino acid residues, which contain a nuclear localization motif (NLS), nine casein kinase II motifs (S/T‐X‐X‐D/E) and seven protein kinase C motifs (S/T‐X‐R/K). It is highly homologous to other plant Mlo proteins. Thus, this clone was designated as Ta‐Mlo (GenBank accession No. AF384144). Northern blotting analysis showed that the transcription of Ta‐Mlo was enhanced slightly by Blumeria graminis (DC) EO Speer f. sp. tritici. Western blotting analysis showed that the protein expression product of the Ta‐Mlo gene in wheat seedling leaves is a membrane‐bound protein. The protein could be induced by B. graminis. Southern blotting analysis indicated that there is one copy of the Ta‐Mlo gene in each wheat genome. Ta‐Mlo was localized on specific chromosomal regions of 2AL, 2BL, and 2DL in wheat. (Managing editor: Li‐Hui ZHAO)