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OsHT , a Rice Gene Encoding for a Plasma‐Membrane Localized Histidine Transporter
Author(s) -
LIU Di,
GONG Wei,
BAI Yong,
LUO JingChu,
ZHU YuXian
Publication year - 2005
Publication title -
journal of integrative plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.734
H-Index - 83
eISSN - 1744-7909
pISSN - 1672-9072
DOI - 10.1111/j.1744-7909.2005.00016.x
Subject(s) - histidine , complementary dna , arabidopsis , amino acid , amino acid transporter , transmembrane domain , biology , biochemistry , peptide sequence , gene , cdna library , saccharomyces cerevisiae , transporter , open reading frame , microbiology and biotechnology , mutant
Using a degenerative probe designed according to the most conservative region of a known Lys‐ and His‐specific amino acid transporter (LHT1) from Arabidopsis , we isolated a full‐length cDNA named OxHT (histidine transporter of Oryza sativa L.) by screening the rice cDNA library. The cDNA is 1.3 kb in length and the open reading frame encodes for a 441 amino acid protein with a calculated molecular mass of 49 kDa. Multiple sequence alignments showed that OsHT shares a high degree of sequence conservation at the deduced amino acid level with the Arabidopsis LHT1 and six putative lysine and histidine transporters. Computational analysis indicated that OsHT is an integral membrane protein with 11 putative transmembrane helices. This was confirmed by the transient expression assay because the OsHT‐GFP fusion protein was, indeed, localized mainly in the plasma membrane of onion epidermal cells. Functional complementation experiments demonstrated that OsHT was able to work as a histidine transporter in Saccharomyces cerevisiae , suggesting that OsHT is a gene that encodes for a histidine transporter from rice. This is the first time that an LHT‐type amino acid transporter gene has been cloned from higher plants other than Arabidopsis. ( Managing editor: Li‐Hui ZHAO)