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Physiological and yield responses of recombinant chromosome substitution lines of barley to terminal drought in a M editerranean‐type environment
Author(s) -
del Pozo A.,
Castillo D.,
Inostroza L.,
Matus I.,
Méndez A.M.,
Morcuende R.
Publication year - 2012
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.2011.00528.x
Subject(s) - biology , hordeum vulgare , anthesis , agronomy , introgression , growing season , poaceae , stomatal conductance , drought tolerance , irrigation , field experiment , botany , cultivar , gene , photosynthesis , genetics
Physiological traits and productivity of the recombinant chromosome substitution lines ( RCSLs ) of barley, developed through the cross of Hordeum vulgare ssp. v ulgare cv. Harrington and the wild ancestor Hordeum vulgare ssp. spontaneum , were measured in plants growing in microplots (with and without irrigation) and in field conditions in two M editerranean‐type environments, C auquenes (rainfed) and S anta R osa (irrigated). The objectives were to assess the degree of phenotypic variability in response to terminal drought stress and to test whether the introgression of the wild ancestor into cv. Harrington can increase the terminal drought tolerance of RCSLs of barley. Days from emergence to anthesis and from anthesis to maturity of the 80 RCSLs were reduced in only 2–4 days under water stress, in microplots. Specific leaf area ( SLA ) and stomatal conductance ( gs ) of 80 RCSLs and cv. Harrington decreased greatly under water stress in plants growing in microplots and field conditions (in 2004/05 growing season). No G × E interaction was detected except for SLA in the microplot experiment. The principal component analysis provided a clear distinction between RCSLs . Along the first principal component, it was possible to identify 24 RCSLs which represent the whole range of grain yield ( GY ), gs and SLA observed in the 80 RCSLs . The selected 24 RCSLs were evaluated in field conditions at C auquenes and S anta R osa, during two growing seasons (2007/08 and 2008/09). The gs and carbon isotope discrimination in grains (Δ 13 C ) were significantly ( P < 0.001) lower in the rainfed condition ( C auquenes), but the water‐soluble carbohydrates ( WSC ) in stems at anthesis and maturity was significantly ( P < 0.001) higher than in well‐irrigated condition ( S anta R osa). Grain yield was reduced by 63% under drought conditions. Differences between RCSLs in gs, WSC and GY were significant ( P < 0.001) in 2007/08. The stress tolerance index ( STI ) was highly ( P < 0.01) correlated with GY in all environments (rainfed and irrigated conditions and the two growing seasons). The relationship between STI and Δ 13 C under rainfed condition allowed identifying drought tolerant and susceptible RCSLs ; the former were high yielding lines under rainfed and irrigated conditions (and higher STI values), but with similar GY to cv. Harrington, but presented higher grain Δ 13 C values than cv. Harrington. The drought susceptible lines presented lower GY , STI and Δ 13 C values than cv. Harrington. These results suggest that H. spontaneum has contributed alleles that increase terminal drought tolerance to some of the RCSLs .