Premium
Mechanism of strong resistance to Potato leafroll virus infection in a clone of potato ( Solanum tuberosum )
Author(s) -
SolomonBlackburn R.M.,
Nikan J.,
Barker H
Publication year - 2008
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.2008.00223.x
Subject(s) - biology , potato leafroll virus , myzus persicae , inoculation , solanum tuberosum , luteovirus , solanaceae , clone (java method) , aphis gossypii , virus , plant virus , virology , horticulture , botany , homoptera , aphid , aphididae , pest analysis , gene , biochemistry
The Solanum tuberosum clone G8107(1) is highly resistant to Potato leafroll virus (PLRV) accumulation following graft inoculation and to PLRV infection by aphids. In glasshouse/insectary experiments, plants were inoculated with viruliferous aphids ( Myzus persicae ); infection was detectable in some inoculated leaves (by enzyme‐linked immunosorbent assay or reverse transcription polymerase chain reaction) but not in petioles, stems or non‐inoculated leaves of inoculated plants. Secondary infection in tuber‐progeny plants of inoculated plants was rare in G8107(1) (detected in only one of 86 tuber‐progeny plants of 24 inoculated plants), while universal in susceptible controls. This resistance did not break down when tuber sprouts were inoculated or when a more efficient M. persicae vector clone was used. Aphids were able to acquire and transmit PLRV infection from infected plants of G8107(1) to test plants but at a lower frequency than from the susceptible cv. Maris Piper. We suggest that an important element of the very strong resistance of this clone to PLRV infection is resistance to virus movement within leaves or from leaves to petioles, thus preventing spread to the rest of the plant.