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Amplified fragment length polymorphism among Rhynchosporium secalis isolates collected from a single barley field in Syria
Author(s) -
KIROSMELES A,
UDUPA S,
ABANG M M,
ABUBLAN H,
BAUM M,
CECCARELLI S,
YAHYAOUI A H
Publication year - 2005
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.2005.040139.x
Subject(s) - biology , amplified fragment length polymorphism , genetic diversity , population , genetic variation , hordeum vulgare , genetics , genetic variability , botany , genotype , poaceae , gene , demography , sociology
Summary AFLP markers were used to measure the amount and distribution of genetic variation among Rhynchosporium secalis isolates on a microgeographical scale in Syria. Forty isolates hierarchically sampled from a single barley field were assayed for AFLP variation using primer combinations not previously tested in populations of the pathogen from Syria. In contrast to a previous study, which showed high clonality within field populations of R. secalis in Syria, the present study revealed a much higher level of genetic diversity, stressing the important roles that sampling strategies and the choice of primers/primer combinations play in the evaluation of genetic variation in R. secalis populations at a microgeographical scale. A high level of genetic variation was found to occur on a fine scale throughout the pathogen population examined, with 40 different haplotypes being identified among the 40 isolates sampled. Data were consistent with the hypothesis that the primary inoculum originated from a genetically diverse founding population, which may have consisted of ascospores of an as yet undescribed teleomorph and/or asexual spores of a highly mutable local population.