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Agrobacterium tumefaciens‐mediated transformation and regeneration of herbicide resistant onion ( Allium cepa ) plants
Author(s) -
EADY COLIN,
DAVIS SHEREE,
FARRANT JULIE,
READER JILL,
KENEL FERNAND
Publication year - 2003
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.2003.tb00243.x
Subject(s) - allium , biology , cultivar , transformation (genetics) , agrobacterium tumefaciens , glyphosate , liliaceae , botany , horticulture , agronomy , gene , biochemistry
Summary Transgenic onion plants ( Allium cepa ) tolerant to herbicides containing active ingredients glyphosate and phosphinothricin were recovered from immature embryos of open pollinated and hybrid parent onion lines at a maximum transformation frequency of 0.9%. Transformants of different onion cultivars, grown on different selective agents and confirmed by Southern analysis, thrived with no apparent ill effects when sprayed with the respective herbicides at double the recommended field dosage for weed eradication. This study demonstrates that the transformation process described previously can be used with different selective agents and is cultivar independent.