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Patchouli virus X, a new potexvirus from Pogostemon clabin
Author(s) -
FILHO P E MEISSNER,
RESENDE R de O,
LIMA M I,
KITAJIMA E W
Publication year - 2002
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.2002.tb00218.x
Subject(s) - potexvirus , biology , virology , potato virus x , virus , mottle , nepovirus , plant virus , myzus persicae , nicotiana benthamiana , botany , rna , coat protein , biochemistry , aphid , gene
Summary This work describes a potexvirus obtained from patchouli, Pogostemon clabin , collected in São Paulo, Brazil in 1992. The plants showed mosaic and were infected by a potyvirus and a potexvirus. The potexvirus had a host range limited to Amaranthaceae, Solanaceae and Labiatae and was named Patchouli virus X (PatVX). PatVX was not transmitted by scissors pruning, in tobacco seeds or by Myzus nicotinae. The virus was purified and a specific antiserum with a titre great than 1:512 000 in dot‐ELISA was produced. The virus was serologically related to Papaya mosaic virus, Potato virus X , Viola mottle virus, White clover mosaic virus and Lily virus X. It had a coat protein of 21 071 ± 1 010 Mr. as determined by SDS‐PAGE. Immunolabelling tests demonstrated that fibrillar masses in the cytoplasm contain the coat protein. The presence of a dsRNA was detected in PatVX infected plants.