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Genetic Manipulation of Rubisco: Chromatium vinosum rbc L is expressed in Nicotiana tabacum but does not form a functional protein
Author(s) -
MADGWICK P J,
COLLIVER S P,
BANKS F M,
HABASH D Z,
DULIEU H,
PARRY M A J,
PAUL M J
Publication year - 2002
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.2002.tb00152.x
Subject(s) - rubisco , biology , nicotiana tabacum , chloroplast , gene , transit peptide , plasmid , genetics , manduca sexta , microbiology and biotechnology , biochemistry , botany , insect , plastid
Summary N. tabacum lines that lacked functional Rubisco were transformed with plasmids encoding a chloroplast transit peptide in frame with C. vinosum rbc L and stable transformants generated. However, the transgene was transcribed at a low level and no Rubisco activity or C. vinosum large subunits were detectable in any line.