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Genetic variation in native and introduced populations of the ‘New Zealand flatworm’, Arthurdendyus triangulatus
Author(s) -
DYNES C,
FLEMING C C,
MURCHIE A K
Publication year - 2001
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.2001.tb00393.x
Subject(s) - biology , flatworm , intraspecific competition , genetic variation , genetic distance , geographical distance , restriction fragment length polymorphism , phylogenetic tree , population , evolutionary biology , internal transcribed spacer , analysis of molecular variance , population genetics , ecology , genetics , zoology , polymerase chain reaction , genetic structure , gene , demography , sociology
Summary The internal transcribed spacer regions (ITS1 and ITS2) including the 5.8S region of the ‘New Zealand flatworm’, Arthurdendyus triangulates , are 1004 base pairs in length. Restriction fragment length polymorphism analysis of PCR products (PCR‐RFLP) was conducted on A. triangulates specimens from 45 locations in Northern Ireland, Scotland, England and New Zealand. Seven restriction endonucleases (Alu I, Rsa I, Sau3A I, Cfo I, Nde I, Dde I, and Mbo I) were used to reveal intraspecific variation. Analysis of molecular variance revealed the presence of population genetic substructuring, with most genetic heterogeneity present between populations rather than between individuals or geographical regions. No distinct differences were found between Northern Irish and Scottish populations but phylogenetic analysis supports the hypothesis of multiple introductions from New Zealand. There was no significant relationship between genetic distance and geographic distance, as would be expected for natural spread, indicating that this species is largely anthropochorous, even in parts of New Zealand.