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Evaluation of some garlic (Allium sativum L.) mutants resistant to white rot disease by RAPD analysis
Author(s) -
NABULSI I,
ALSAFADI B,
ALI N MIR,
ARABI M I E
Publication year - 2001
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.2001.tb00102.x
Subject(s) - biology , rapd , allium sativum , sclerotium , mutant , allium , stem rot , genetic diversity , genetics , primer (cosmetics) , sativum , botany , gene , population , chemistry , demography , organic chemistry , sociology
Summary Random amplified polymorphic DNA (RAPD) analysis was used to evaluate genetic diversity among eight garlic mutants resistant to white rot disease ( Sclerotium cepivorum ). Twelve of the 13 synthetic random primers were found to identify polymorphism in amplification products. Mutants characterised with moderate resistance to white rot were closely related to the control using cluster and correlation analyses. On the other hand, highly resistant mutants were quite distant from the control with low correlation coefficients. The banding patterns produced by primer OPB‐15 (GGAGGGTGTT) with highly resistant mutants may by used as genetic markers for early selection of resistant plants.