Premium
Improved purification and molecular properties of Omithogalum mosaic virus in Israel
Author(s) -
ZEIDAN M.,
COHEN J.,
WATAD A.,
GERA A.
Publication year - 1998
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1998.tb05817.x
Subject(s) - biology , virus , virology , open reading frame , potyvirus , antiserum , polymerase chain reaction , microbiology and biotechnology , nucleotide , complementary dna , rna , plant virus , peptide sequence , gene , biochemistry , genetics , antibody
Summary. Symptoms associated with infection by Omithogalum mosaic virus (OMV) were documented among horticulturally important Omithogalum spp. grown in commercial greenhouses and open fields in Israel. OMV was mechanically transmitted to Chenopodium quinoa and C. amaranticolor. The virus was purified from infected C. quinoa using CsCl step gradient centrifugation. Virus yield of 18–24 mg kg“ 1 were obtained. Antiserum produced to the purified virus was highly specific in immunoblots and immunosorbent electron microscopy. Reverse transcription ‐ polymerase chain reaction (RT‐PCR) utilising potyvirus‐specific primers and the viral RNA resulted in the amplification of a DNA product of 1275 nucleotides. This PCR product was cloned and sequenced; it encoded an open reading frame of 333 codons and a 3‘ non‐coding region of 275 nucleotides. Analysis of the amino acid sequences of the putative CP of the Israeli isolate of OMV showed 95% similarities to the South African isolate.