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Distribution of Burkholderia cepacia phenotypes by niche, method of isolaiton and pathogenicity of onion
Author(s) -
YOHALEM DAVID S.,
LORBEER JAMES W.
Publication year - 1997
Publication title -
annals of applied biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 80
eISSN - 1744-7348
pISSN - 0003-4746
DOI - 10.1111/j.1744-7348.1997.tb07675.x
Subject(s) - burkholderia , biology , pathogenicity , isolation (microbiology) , microbiology and biotechnology , strain (injury) , bacteria , pseudomonas , genetics , anatomy
Summary Several proposals have been made for the subdivision of the bacterial species Burkholderia cepacia . Data applying these schemes to a collection of 188 strains of B. cepacia , isolated using a variety of methods, from soils, decayed onions, and nosocomial sources are presented. None of the schemes could be shown to be associated with the method of isolation, niche from which a strain was isolated, pectolytic activity, or pathogenicity to onion. Strains isolated by serial dilution on onion slices were much more uniform than were strains isolated using semiselective media. The nutritionally stringent medium of Lumsden and Sasser (US Patent No. 4 588 584) was judged better for the collection of a phenotypically broad sample of B. cepacia than those of Hagedorn, Gould, Bardinelli & Gustavson (1987) and Wu & Thompson (1984), which are based on antibiotic insensitivity. Pectolytic activity and pathogenicity to onion were shown to be highly correlated characters. None of the strains of clinical origin was capable of macerating onion tissue.